Areas of major interest in cell culture

In order to keep cell cultures healthy and actively growing it is necessary to subculture them carefully at regular intervals.

The characteristics of the human, animal and hybridoma cell lines available in our stock are included in the following Tables.

Human Cell Lines

Cell line	Cell type	Morphology
HL-60	acute myeloid leukemia	round, single cells in suspension
K-562	chronic myeloid leukemia in blast crisis	round large, single cells in suspension
REH	B cell precursor leukemia	small, round, single cells in suspension
DG-75	Burkitt's lymphoma	round to polygonal, single or clustered cells in suspension
RAJI	Burkitt's lymphoma	round single or clustered cells in suspension
JURKAT	T cell leukemia	round cells growing singly or in clumps in suspension
NC-37	peripheral blood lymphocyte	lymphoblast
A-549	lung carcinoma	epithelial cells, growing adherently as monolayers
CCL-205	lung; fibroblast; normal	epithelial adherent
MRC-9	fetal lung	fibroblast
DU-145	prostate carcinoma	epithelial-like adherent cells growing as monolayers
HELA	cervix carcinoma	epithelial-like cells growing in monolayers
HTB-22	breast adenocarcinoma	epithelial-like cells growing as monolayers
HTB-121	breast; ductal carcinoma	epithelial adherent
HTB-125	normal breast tissue peripheral to an infiltrating ductal carcinoma	epithelial adherent
MDA-MB-453	breast carcinoma	epithelial-like, round, loosely attached monolayers
A-431	epidermoid carcinoma	epithelial-like, adherent cell line growing in monolayers
NK-92	natural killer lymphoma	cells growing in clumps in suspension
HaCat	immortalized keratinocytes	adherent

Animal Cell Lines

Cell line	Cell type	Morphology

AR42J	rat exocrine pancreatic tumour	pancreas cells
X63Ag8.653	mouse myeloma	single, round, large cells in suspension
L-929	mouse connective tissue fibroblast	fibroblasts growing as monolayer
3T3	Swiss albino mouse fibroblasts	fibroblasts growing adherently as monolayer with contact inhibition
P-388D1	mouse monocytes-macrophages	adherent elliptical and round loosely adherent cells growing in monolayers
COS-7	African green monkey kidney	fibroblast-like cells growing as monolayers
CHO-K1	Chinese hamster ovary cells	adherent, fibroblastoid cells
MDCK	dog Cocker Spaniel kidney	epithelial
Ptk2	potoroo kidney	epithelial-like

Hybridomas and monoclonal antibodies

Members

Cell type