Scope:
Our aim is to
investigate the intrinsic DNA damage in humans. Intra-( and
interindividual variations were detected dependent on the
(patho)-physiological state in healthy volunteers. Additionally we
measure individual repair capacities towards external oxidative stress.
To access these DNA damage and repair activities we apply an adopted
Comet-assay protocol. Our aim is to study whether there is an age
correlation or not to intrinsic or induced oxidative damages.
Methods:
We use a modified
neutral, detergent based lysis protocol of the Comet-assay.
Additionally Comet-FISH is used to study the specific DNA
damage in selected whole chromosomes.
Sample images
Results:Sample images
We have monitored 14
individuals over a time period of more than 18 months. Among our test
individuals some showed a stable genome while others revealed an
unstable genome. Also we found that external pertubations of the normal
living habits affects the detected level of DNA damages.
Short term observations
Long term observations
Taken together no dependence of the DNA fragmentation was found on the age, but strong inter individual differences.
Age dependence of intrinsic and induced DNA damage
Further perspectives:Short term observations
Long term observations
Taken together no dependence of the DNA fragmentation was found on the age, but strong inter individual differences.
Age dependence of intrinsic and induced DNA damage
At the moment we are
setting up a siRNA based in vitro cell culture system to mimic partial
reduced enzymatic activity and their effects on cellular response
towards genotoxic stress.
| DNA
Damage and Repair Topics |
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| UV-A
Induced DNA Damage |
DNA Double Strand Repair | DNA Repair Home |
Comet-assay &
Comet-FISH |