Head

Karl Otto Greulich

Group members

Franziska Jahns

Anne Wilhelm

Former lab members

Konrad Böhm

Kerstin Dreblow

Paulius Grigaravicius

Gabriele Günther

Kornelia Haus (secretary)

Nadja Jablonowski

Stefanie Kretschmar

Shamci Monajembashi

Maria Radeva

Marina Wollmann

Leo Wollweber

Eberhard Unger



 

Greulich Laboratory

Karl-Otto Greulich has retired as FLI Group Leader in March 2012. He is still affiliated with the institute as guest scientist.


for details look here on the lab's web pages

 

Ubiquitous cancer genes, dsDNA repair and wound repair in aging and cancer

Ubiquitous cancer genes, dsDNA repair and wound repair in aging and cancer

Ubiquitous cancer genes, dsDNA repair and wound repair in aging and cancer

Ubiquitous cancer genes, dsDNA repair and wound repair in aging and cancer

Ubiquitous cancer genes, dsDNA repair and wound repair in aging and cancer

Ubiquitous cancer genes, dsDNA repair and wound repair in aging and cancer

Research interests

  • The earliest events in DNA repair
  • Gene versus protein expression in ageing and colon cancer
  • Multitissue studies on changing gene expression in cancers
  • The nature of light and the wave particle dualism

 

Major recent achievements

  • XRCC4 comes surprisingly early in ds DNA repair
  • gene expression does not predict protein expression
  • surprisingly few genes are affected in a majority of human cancer tissues
  • blocking - proteins break down neuronal transport and may be involved in neurodegenerative diseases

    invited editorship (2007) in the US book series 'Methods in Cell Biology'
    report to the public in 'Die Welt' of 7. May 2008

 

Contribution to FLI's overall mission

  • DNA repair in the context of ageing
  • individual expression differences in colon cancer, a disease of the elderly
  • age dependent gene expression in human individuals
  • role of intracellular transport along microtubules in neurodegenerative disease

 

Recent selected publications

  • Greulich KO, Grigaravicius, Monajembashi S (2012) Single DNA molecule typing, heart beating, DNA repair and ageing: the contribution of fluorescence techniques. Reviews in Fluorescence, 2010, 199-222. [Springer]
  • Jahns F, Wilhelm A, Greulich KO, Mothes H, Radeva M, Wölfert A, Glei M (2012) Impact of butyrate on PKM2 and HSP90ß expression in human colon tissues of different transformation stages: a comparison of gene and protein data. Genes Nutr, 7, 235-246. [PubMed]
  • Kuhnert A, Schmidt U, Monajembashi S, Franke C, Schlott B, Grosse F, Greulich KO, Saluz HP, Hänel F (2012) Proteomic identification of PSF and p54(nrb) as TopBP1-interacting proteins. J Cell Biochem, 113, 1744-1753. [PubMed]
  • Wilhelm A, Jahns F, Böcker S, Mothes H, Greulich KO, Glei M (2012) Culturing explanted colon crypts highly improves viability of primary non-transformed human colon epithelial cells. Toxicol in Vitro, 26, 133-141. [PubMed]
  • Altenberg B, Greulich KO (2011) Laser microbeam – kinetic studies combined with molecule – structures reveal mechanisms of DNA repair. Proc SPIE, 8097, 80970D. [Proceedings]
  • Greulich KO (2011) Photons bring light into DNA repair: the comet assay and laser microbeams for studying photogenotoxicity of drugs and ageing. J Biophotonics, 4, 165-171. [PubMed]
  • Jahns F, Wilhelm A, Jablonowski N, Mothes H, Radeva M, Wölfert A, Greulich KO, Glei M (2011) Butyrate suppresses mRNA increase of osteopontin and cyclooxygenase-2 in human colon tumor tissue. Carcinogenesis, 32, 913-920. [PubMed]
  • Greulich KO (2010) Calculation of the masses of all fundamental elementary particles with an accuracy of approx. 1%. J Mod Phys, 1, 300-302. [Journal]
  • Greulich KO (2010) Optical Tweezers. Encyclopedia of Life Sciences. Elsevier, doi: 10.1002/9780470015902.a0003038.pub2. [Website]
  • Greulich KO (2010) Single molecule experiments challenge the strict wave-particle dualism of light. Int J Mol Sci, 11, 304-311. [PubMed]
  • Radeva MY, Jahns F, Wilhelm A, Glei M, Settmacher U, Greulich KO, Mothes H (2010) Defensin alpha 6 (DEFA 6) overexpression threshold of over 60 fold can distinguish between adenoma and fully blown colon carcinoma in individual patients. BMC Cancer, 10, 588. [PubMed]
  • Grigaravicius P, Greulich KO, Monajembashi S (2009) Laser microbeams and optical tweezers in ageing research. ChemPhysChem, 10, 79-85. [PubMed]
  • Grigaravicius P, Rapp A, Greulich KO (2009) A direct view by immunofluorescent comet assay (IFCA) of DNA damage induced by nicking and cutting enzymes, ionizing 137 Cs radiation, UV A laser microbeam irradiation and the radiomimetic drug bleomycin. Mutagenesis, 24, 191-197. [PubMed]
  • Greulich KO (2008) The wave property of heavy molecules - Its use in mass spectrometry. Angew Chem Int Ed Engl, 47, 7990-7991. [PubMed]
  • Berns MW, Greulich KO (Eds) Laser Manipulation of Cells and Tissue. Methods in Cell Biology (2007) Vol 82, Elsevier, Academic Press. [Amazon]
  • Altenberg B, Gemuend C, Greulich KO (2006) Ubiquitous cancer genes: multipurpose molecules for protein micro-arrays. Proteomics, 6, 67-71. [PubMed]
  • Altenberg B, Greulich KO (2004) Genes of glycolysis are ubiquitously overexpressed in 24 cancer classes. Genomics, 84, 1014-1020. [PubMed]
  • Rapp A, Greulich KO (2004) After double-strand break induction by UV-A, homologous recombination and nonhomologous end joining cooperate at the same DSB if both systems are available. J Cell Sci, 117, 4935-4945. [PubMed]
  • Rapp A, Bock C, Dittmar H, Greulich KO (2000) UV-A breakage sensitivity of human chromosomes measured by COMET-FISH depends on gene density and not on the chromosome size. J Photochem Photobiol B, 56, 109-117. [PubMed]
  • Greulich KO (Ed) Micromanipulation by Light in Biology and Medicine: the Laser Microbeam and Optical Tweezers. Methods in Bioengineering (1999), Birkhäuser Boston. [Amazon]

 

Last update: April 19, 2012
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