Imaging Facility - Hemmerich Laboratory

Imaging Facility

The Imaging Facility of the FLI provides the microscopy infrastructure to support state of the art research in biological sciences. It maintains several light, fluorescence and advanced confocal microscopes, and provides introductions and service for FLI researchers. External users may access the Imaging Facility through collaborations with FLI research groups. A list of microscopes can be found (not yet) here. The FLI Imaging Facility is part of the network of Biological Imaging Facilities in Germany.

Research

We are studying the structure and function of the cell nucleus. With respect to cellular ageing and age-related diseases we are interested in senescence-related alterations within the nucleus, including chromatin dynamics and promyelocytic leukemia (PML) nuclear body function. Our research on cellular senescence is embedded in the JenAge Project.

Selected publications

Marthandan S, Murphy MP, Billett E, Barnett Y (2011) An investigation of the effects of MitoQ on human peripheral mononuclear cells. Free Radic Res, 45, 351-358. [PubMed]
Hemmerich P, Schmiedeberg L, Diekmann S (2011) Dynamic as well as stable protein interactions contribute to genome function and maintenance. Chromosome Res, 19, 131-151 [PubMed]
Larbi A, Cabreiro F, Zelba H, Marthandan S, Combet E, Friguet B, Petropoulos I, Barnett Y, Pawelec G (2010) Reduced oxygen tension results in reduced human T cell proliferation and increased intracellular oxidative damage and susceptibility to apoptosis upon activation. Free Radic Biol Med, 48, 26-34. [PubMed]
Lenser T, Weisshart K, Ulbricht T, Klement K, Hemmerich P (2010) Fluorescence fluctuation microscopy to reveal 3D architecture and function in the cell nucleus. Methods Cell Biol, 98, 2-33 [PubMed]
Brand P, Lenser T, Hemmerich P (2010) Assembly dynamics of PML nuclear bodies in living cells. PMC Biophys. , 3, 3 [PubMed]
Lang M, Jegou T, Chung I, Richter K, Münch S, Udvarhelyi A, Cremer C, Hemmerich P, Engelhardt J, Hell SW, Rippe K (2010) Three-dimensional organization of promyelocytic leukemia nuclear bodies. J Cell Sci, 123, 392-400 [PubMed]
Weidtkamp-Peters S, Weisshart K, Schmiedeberg L, Hemmerich P (2009) Fluorescence correlation spectroscopy to assess the mobility of nuclear proteins. Methods Mol Biol, 464, 321-341 [PubMed]
Weidtkamp-Peters S, Lenser T, Negorev D, Gerstner N, Hofmann TG, Schwanitz G, Hoischen C, Maul G, Dittrich P, Hemmerich P (2008) Dynamics of component exchange at PML nuclear bodies. J Cell Sci, 121, 2731-2743 [PubMed]
Murzik U, Hemmerich P, Weidtkamp-Peters S, Ulbricht T, Bussen W, Hentschel J, von Eggeling F, Melle C (2008) Rad54B targeting to DNA double-strand break repair sites requires complex formation with S100A11. Mol Biol Cell, 19, 2926-2935 [PubMed]
Schmiedeberg L, Weisshart K, Diekmann S, Meyer zu Hoerste G, Hemmerich P (2004) High and low mobility populations of HP1 in heterochromatin of mammalian cells. Mol Biol Cell, 15, 2819-2833 [PubMed]
Wieland G, Orthaus S, Ohndorf S, Diekmann S, Hemmerich P (2004) Functional complementation of human centromere protein A (CENP-A) by Cse4p from Saccharomyces cerevisiae. Mol Cell Biol, 24, 6620-6630 [PubMed]
Kießlich A, v. Mikecz A, Hemmerich P (2002) Cell Cycle-dependent association of PML bodies with sites of active transcription. J Struct Biol, 140, 167-179 [PubMed]
v. Mikecz A, Zhang S, Montminy M, Tan E, Hemmerich P (2002) CREB-binding protein (CBP)/p300 and RNA polymerase II colocalize in transcriptionally active domains in the nucleus. J Cell Biol, 150, 265-273 [PubMed]
Hemmerich P, Stoyan T, Wieland G, Koch M, Lechner J, Diekmann S (2002) Interaction of yeast kinetochor proteins with centromere-protein/transcription factor Cbf1. Proc Natl Acad Sci U S A, 97, 12583-12588 [PubMed]

Last update: June 6, 2011

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