Single Molecule Enzyme Kinetics

The kinetics of the reaction Lactate + NAD+ <---> NADH + Pyruvate + H+ could be measured while the reaction was catalysed by single lactate dehydrogenase enzyme molecules. The image shows clouds of fluorescent NADH (reaction zones) around single enzyme molecules. single molecule enzyme reactionClick the image to see a larger panel of this reaction. 

Click here to view an animated gif of the reaction (700k, set your monitor to 256 colors or more).



 Single Molecule Topic
Single Molecule Research Overview
Single Molecule DNA Handling