The
kinetics of the reaction Lactate + NAD+
<---> NADH + Pyruvate + H+ could be measured while the
reaction
was catalysed by single lactate dehydrogenase enzyme molecules. The
image
shows clouds of fluorescent NADH (reaction zones) around single enzyme
molecules.
Click
the image to see a larger panel of this reaction.
Click
here
to view an animated gif of the reaction (700k, set your monitor to 256
colors or more).