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  Microtubule Lab

Common Research Interests: Cell biology and biochemistry of microtubules and kinesin

• Expression of recombinant kinesins (KIF5A, Eg5)

• Investigation of kinesin-dependent microtubule motility in vitro (gliding assay, bead assay)

• Purification of microtubule proteins (tubulin, MAP1, MAP2, tau) from mammalian brain

• In vitro assembly of microtubule proteins under the influence of effectors (drugs, signalling proteins, lipopolysaccharides)

• Transmission electron microscopy of in vitro reconstituted protein assemblies

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Electron microscopy of microtubules

Kinesin-mediated motility in cell-free environment

• Kinesin-driven microtubule gliding

• Isopolar orientation of gliding microtubules in a flow field

• Kinesin-driven particle transport along isopolar microtubules

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Research techniques

• Cell homogenization and preparative ultracentrifugation

• Protein purification (affinity chromatography, gel filtration, ultrafiltration)

• Transmission electron microscopy including ultrathin sectioning and whole-mount sample preparation

• Video-enhanced differential interference contrast (AVEC-DIC) microscopy

• Time-dependent turbidity measurement

• Immunofluorescence microscopy, immunogold labelling

• SDS polyacrylamide gel electrophoresis and blotting techniques

• ATPase and GTP activity measurements

Links to cytoskeleton groups

• Microtubule Labs

• Kinesin Labs (Kinesin People)

Go to:        Institute For Age Research - Fritz Lipmann Institute

Go to:        Molecular Motors

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