Microtubules visualized by video-enhanced differential interference contrast microscopy

mtvideo.jpg

Microtubules assembled in vitro from tubulin in the presence of microtubule-associated proteins, bound to glass slides.


Kinesin-driven microtubule gliding

Kinesin-driven microtubule gliding on glass surfaces in vitro.  Mean gliding velocity: 0.6 - 0.8 µm/s.
 

References:
Böhm K.J. et al.: Speeding up kinesin-driven microtubule gliding in vitro by variation of cofactor composition and physicochemical parameters.
        Cell Biology International  24, 335-341 (2000)

Böhm K.J. et al.: Kinesin-driven microtubule motility in the presence of alkaline-earth metal ions. Indication for calcium ion-dependent motility.
        Cell Motility and Cytoskeleton 37, 226-231 (1997)

Back to Konrad´s Home Page